Continuous and perfusion culture of suspension and adherent mammalian cells

Featuring 1.5 days of theory and 2 days of hands-on practice, this training program focuses on culturing adherent and suspension-adapted mammalian cell lines to produce a monoclonal IgG. Cost: 3,300 CHF

Learning objectives

• We will perform perfusion cultures in different types of bioreactor configurations.
• Perfusion will be achieved by a variety of cell recycle techniques, including acoustic wave separation.
• Adherent cells will be grown in fixed-bed or fluidized-bed reactors with different types of micro-carriers.
• We will examine the optimization of growth and production kinetics.
• We will demonstrate how continuous USP can be integrated to the first stage of DSP to enable development of completely continuous bioprocesses.

Who should attend?

This course is for people having a basic knowledge of cell culture, but wish to explore or develop their knowledge in high-productivity perfusion systems. This can be stand-alone or combined to create completely continuous production processes. People interested at all levels, whether academic, research or industrial, in the production of therapeutic recombinant proteins, vaccines or viral vectors should attend. The course is available for biologists, engineers and process developers who wish to gain at in-depth understanding of the methods that can be used to create an efficient, robust perfusion process.